Culture and maintenance of Agrobacterium strains.

Influence of different strains of Agrobacterium rhizogenes, culture medium, age and type of explant on hairy root induction in Echinacea angustifolia

Hairy roots culture is a potential system for secondary metabolite production in medicinal plants. In this research, hairy root induction in Echinacea angustifolia was established using Agrobacterium rhizogenes. The effects of dif-ferent A. rhizogenes strains (AR15834, A4, K599 and MSU440) in two solid growth regula-tor-free culture media, half strength MS salts with B5 vitamins and WPM, were e...

Evaluation of Effect of β-Lactam Antibiotics on Suppression of Different Strains of Agrobacterium tumefaciens and on Wheat Mature Embryo Culture

Hairy Roots Formation in Four Solanaceae Species by Different Strains of Agrobacterium rhizogenes

Plants are the important sources of drugs. Secondary metabolites are responsible for therapeutic propertiesin plants. Three compounds including (-)-hyoscyamine, its racemate atropine, and scopolamine (hyoscine) are the most famous tropane alkaloids in the Solanaceae familly. Nowadays, attempts to developthese alkaloids in biotechnological procedures which are principally based on the hairy root...

بررسی برخی فاکتورهای موثر در انتقال ژن به گیاه دوپایه ترشک ( Rumex acetosa L )

Sorrel (Rumex acetosa L.) is a model dioecious plant in genetic and molecular studies for sex determination. In this research, gus reporter gene transformation to leaf disks of this plant via Agrobacterium tumefaciens was evaluated based on transient expression of this gene. Three strains of Agrobacterium( LBA4404, C58 and EHA101) and two kinds of bacterial suspensions (suspension I: Agrobacter...

Plasmid-dependent attachment of Agrobacterium tumefaciens to plant tissue culture cells.

Kinetic, microscopic, and biochemical studies show that virulent Ti (tumor inducing)-plasmid-containing strains of Agrobacterium attach to normal tobacco and carrot tissue culture cells. Kinetic studies showed that virulent strains of A. tumefaciens attach to the plant tissue culture cells in increasing numbers during the first 1 to 2 h of incubation of the bacteria with the plant cells. Five T...